Research Assistant - job 2 of 3

Job Summary:

Under general supervision, provides routine technical assistance in performing assigned and delegated tasks in support of research projects.

Job Duties:

1. Molecular Biology: site directed mutagenesis, sequencing and PCR/qRT-PCR. Some training will be provided as necessary. (15%)

2. Biochemical Procedures - Western Blots, immunoblots, SDS-PAGE, quantifying protein concentrations. Some training will be provided as necessary. (20%)

3. General Laboratory Maintenance - preparing agar plates, autoclaving of glassware, media and pipette tips, re-filling purified water and liquid nitrogen dewar. Maintenance of tissue-culture room, incubators, and sterile tissue-culture hoods. Ordering and receiving. (25%)

4. Preparation of primary neurons for culture from rodent peripheral ganglia. Maintenance of tissue-culture cells in tissue-culture room using sterile technique. Transfection of tissue-culture cells. Use of a fluorescent microscope. Animal husbandry. (40%)

Education:

Bachelors in a basic science is required.

Experience:

None