POSITION RESPONSIBILITIES:
The person will:
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Cell processing for functional assays, including, but not limited to:
- Flow cytometry
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Cell culture for assays including cell interaction studies
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Confocal and routine microscopy
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RNA and DNA extraction
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Immunoblotting
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Perform a moderate number of simple, and/or moderate, and/or complex technical procedures;
- Require supervision only rarely;
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Routinely be responsible for ordering supplies;
- May routinely be responsible for requesting quotes and information for purchasing equipment for the laboratory;
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Frequently modify technical procedures;
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Frequently train or guide other lab members;
- May frequently operate, maintain and troubleshoot complex, high tech equipment;
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Frequently modify/formulate experimental protocols;
- Frequently analyze and interpret data;
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May make frequent written contributions to papers and grant proposals;
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An understanding of complex scientific literature and an extensive understanding of research goals and rationale is required.
QUALIFICATIONS:
Hiring Rate: $34.63 Post Probationary Rate: $35.16 Job Rate: $35.88 ABOUT US:
The Rastogi Lab, located in the Pediatric Translational Research facility at Albert Einstein College of Medicine, is focused on the investigation of mechanisms underlying obesity-related asthma in children. We have found that systemic immune responses in obese asthmatic children are skewed to a non-atopic Th1 phenotype, which is associated with metabolic dysregulation. We are now proposing to investigate the mechanisms underlying these non-atopic immune responses.
We are seeking a Lab Technician who is comfortable with processing human samples and are interested in mechanistic studies of chronic diseases including asthma. Under the guidance of Dr. Rastogi, the technician will be responsible for processing of samples collected from children and performing the functional assays, that will include use of flow cytometry, Cell culture for assays including cell interaction studies, confocal microscopy, immunoblotting and DNA and RNA extraction. Expertise in T cell immunology is a major plus.