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Lead - Gene Assembly

Company Description

Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins works with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate.

Eurofins is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. Eurofins is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. The Group also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products.

In over just 30 years, Eurofins has grown from one laboratory in Nantes, France to 55,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 50 countries. Eurofins offers a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products.

Job Description

Since 1999, Eurofins Genomics Blue Heron(Blue Heron Biotech) has been a pioneer in the gene synthesis industry and has delivered tens of millions of base pairs of perfectly accurate genes to thousands of customers worldwide using its proprietary GeneMaker® multi-technology platform. We are 100% committed to serving our customers with high quality, strict confidentiality standards, and rapid delivery of synthesized genes,  Plasmid DNA, and IVTmRNA.

Position Summary:

The Team Leader for Gene Assembly is responsible for performing all activities associated with cloning and high-throughput gene synthesis process (approx. 50%), lead in the investigation and troubleshooting of failed targets (20%), training of new employees (10%), and writing and maintaining the Gene Assembly SOP documents (approx. 10%). The Team Leader for Gene Assembly will also be assigned non-standard assembly projects; and assembly of critical reagents and buffers (10%).

Position Responsibilities/Accountabilities:

  • Clone DNA into expression cloning vectors.
  • Carry out gene cloning steps, including strategy review, enzymatic DNA restriction and modification, PCR primer design and PCR, gel electrophoresis, band isolation and DNA purification, and ligation using standard and proprietary methods.
  • Track and monitor cloning results and follow-up as needed with sequencing, restriction, or PCR analysis.
  • Troubleshoot and remake any failed PCR, ligations, restriction digests, and/or transformations.
  • Review sequencing data for troubleshooting and quality control purposes.
  • Lead by example and encourage team members to their full potential.
  • Perform production protocols according to established company operating procedures and assist with general production support as needed.
  • Work within a multi-disciplinary team; meet goals for accuracy, production deadlines and throughput utilizing the company’s proprietary gene-synthesis platform and related tools.

Qualifications

  • The ideal candidate is self-motivated, possesses outstanding organizational skills, and the demonstrated ability to perform as an effective team member. 
  • He/she will have a strong background in molecular biology with hands-on knowledge of the uses and properties of DNA and enzymes, PCR and thermocyclers, gel electrophoresis, photo documentation, and sequence review.
  • Experience utilizing and manipulating data via databases is preferred. 
  • Good computer skills, precise attention to detail, record keeping, and adherence to protocols is required. 
  • Experience with liquid handling robots or complex laboratory equipment are highly desirable.   

Additional Information

Schedule:

  • This 8 hr position will require weekend work: Sunday to Thursday or Tuesday to Saturday, 8:00 AM to 5:00 PM or 12:00 PM to 9:00 PM

What we offer:

  • Excellent full time benefits including comprehensive medical coverage, dental, and vision options
  • Life and disability insurance
  • 401(k) with company match
  • Paid vacation and holidays

Eurofins USA Genomic Services is a Disabled and Veteran Equal Employment Opportunity employer.

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Average salary estimate

$70000 / YEARLY (est.)
min
max
$60000K
$80000K

If an employer mentions a salary or salary range on their job, we display it as an "Employer Estimate". If a job has no salary data, Rise displays an estimate if available.

What You Should Know About Lead - Gene Assembly, Eurofins

Join Eurofins Scientific as a Lead - Gene Assembly in Louisville, KY, where you will play a pivotal role in the groundbreaking field of gene synthesis. At Eurofins, a global leader in life sciences, you will be part of a dynamic team that has revolutionized the gene synthesis industry since 1999. Your mission as a Team Leader for Gene Assembly will involve a diverse range of activities, from cloning DNA into expression vectors to troubleshooting complex issues in gene synthesis processes. You'll be leading efforts in high-throughput gene assembly and mentoring new team members, ensuring they receive the guidance they need to thrive in our innovative environment. Your passion for molecular biology will be key as you review sequencing data, implement quality control measures, and keep meticulous records adhering to established protocols. With your experience in PCR, gel electrophoresis, and a solid understanding of DNA properties, you'll be at the forefront of leading non-standard assembly projects and producing critical reagents. At Eurofins, we foster a culture of teamwork and excellence, encouraging you to lead by example and help our team members reach their full potential. If you’re looking for a vibrant role with a company that values health, sustainability, and continuous improvement, then the Lead - Gene Assembly position is perfect for you. Dive into this rewarding opportunity and be a vital part of something bigger!

Frequently Asked Questions (FAQs) for Lead - Gene Assembly Role at Eurofins
What are the main responsibilities of the Lead - Gene Assembly at Eurofins Scientific?

The Lead - Gene Assembly at Eurofins Scientific is tasked with various responsibilities, primarily focusing on cloning and high-throughput gene synthesis processes. This includes performing cloning DNA into expression vectors, troubleshooting and investigating failed targets, and leading the training of new employees. Additionally, the role involves writing and maintaining Gene Assembly standard operating procedures, working on assembly projects, and ensuring production protocols are followed accurately.

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What qualifications are needed for the Lead - Gene Assembly position at Eurofins Scientific?

To thrive as a Lead - Gene Assembly at Eurofins Scientific, candidates should possess a strong background in molecular biology, with hands-on experience in DNA and enzyme properties, PCR, and gel electrophoresis. Ideal applicants should also showcase outstanding organizational skills, attention to detail, and an ability to work collaboratively within a team. Experience with liquid handling robots and data manipulation via databases is preferred, highlighting the technical expertise that is crucial for this role.

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What is the work schedule like for the Lead - Gene Assembly at Eurofins Scientific?

The Lead - Gene Assembly position at Eurofins Scientific is structured around an 8-hour work schedule, requiring weekend work. Candidates can expect to work from Sunday to Thursday or Tuesday to Saturday, with shifts scheduled from either 8:00 AM to 5:00 PM or 12:00 PM to 9:00 PM. This scheduling allows for flexibility and ensures coverage across the essential activities in the lab.

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How does the Lead - Gene Assembly contribute to team success at Eurofins Scientific?

As a Lead - Gene Assembly at Eurofins Scientific, you play a vital role in fostering team success by leading by example and encouraging your colleagues to reach their full potential. Your leadership influences the team's productivity and quality of outputs by mentoring new employees and ensuring adherence to established protocols and best practices. By being actively engaged in troubleshooting and guiding team members, you help maintain a high standard of excellence within the Gene Assembly team.

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What career growth opportunities are available for a Lead - Gene Assembly at Eurofins Scientific?

Eurofins Scientific offers various career growth opportunities for its Lead - Gene Assembly positions. Employees are encouraged to pursue professional development through workshops, training, and leadership programs. As Eurofins is a global leader in the life sciences sector, successful performance in this role could lead to advancement into greater leadership positions within the organization, allowing you to shape the future of gene synthesis and laboratory practices.

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Common Interview Questions for Lead - Gene Assembly
Can you describe your experience with gene cloning techniques?

In preparing for your interview, it’s essential to discuss specific gene cloning techniques you’ve utilized, such as restriction digestion, ligation, and transformation. Highlight any successful projects and the outcomes, emphasizing your role in problem-solving during the cloning process. Providing an example where you had to troubleshoot a failed clone will showcase your analytical and critical thinking ability.

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What methods do you use for troubleshooting failed PCR reactions?

When answering this question, detail the steps you take when addressing PCR failures, such as checking reagent quality, optimizing annealing temperatures, and reviewing primer design. Mention any specific experiences where your troubleshooting led to a successful outcome, and discuss how you document these processes to inform future experiments.

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How do you ensure accuracy in your cloning results?

Accuracy can be ensured through meticulous attention to detail and following established protocols rigorously. Discuss your process for double-checking data, running controls, and the importance of sequencing verification. Additionally, share how you maintain comprehensive records of your experiments to facilitate research integrity.

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What experience do you have with liquid handling technologies?

In your response, detail any experience with liquid handling systems, describing how they improve efficiency and accuracy in the lab environment. Talk about specific equipment you’ve operated, such as automated pipetting systems, and cite examples of how your skill in using such technologies has enhanced productivity or reduced error rates in your previous roles.

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Describe a time you had to lead a team under pressure.

Display your leadership qualities by providing an example of a high-pressure situation where you took charge. Discuss how you delegated tasks, rallied your team, and maintained morale while ensuring that project deadlines were met. Emphasize the positive outcomes and lessons learned that you can apply in the position of Lead - Gene Assembly.

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What strategies do you use for training new team members?

Training new team members involves a mix of structured orientation and hands-on guidance. Discuss your approach to onboarding, which might include creating training materials, pairing new hires with mentors, and assessing their progress regularly. Highlight how your strategies ensure that new employees understand their responsibilities and feel supported as they transition into their roles.

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How do you handle project deadlines and prioritize tasks?

In answering this question, illustrate your time management strategies, including how you prioritize responsibilities based on urgency and importance. Share examples of methodologies you utilize, such as task lists or project management software, to stay organized and ensure that team deliverables align with deadlines without compromising quality.

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Can you explain your experience with analyzing sequencing data?

Provide insights into your familiarity with sequencing technologies and data analysis. Talk about specific software tools you’ve used and how you interpret results to ascertain the quality of gene assembly. Discuss any instances where your analysis has directly influenced decisions in cloning projects, showcasing your critical thinking strengths.

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What characteristics do you believe are essential in a team leader?

Discuss attributes such as communication, empathy, decisiveness, and integrity that define an effective team leader. Share your thoughts on how these qualities contribute to a positive team environment and the successful achievement of objectives in the Gene Assembly team.

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Why do you want to work as a Lead - Gene Assembly at Eurofins Scientific?

It's important to convey your genuine enthusiasm for the position and the company. Talk about your alignment with Eurofins' mission of improving health and sustainability through scientific innovation. Express how your skills and experiences make you a great fit for the role and how you’re excited to contribute to their pioneering work in gene synthesis.

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Full-time, on-site
DATE POSTED
March 27, 2025

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