Research Assistant - job 3 of 3
Job Summary:
Under general supervision, provides routine technical assistance in performing assigned and delegated tasks in support of research projects. Responsibilities include assisting or conducting a variety of scientific experiments and research projects of limited complexity, collecting, evaluating, and interpreting data for clinical and diagnostic research.
Job duties:
- Mouse colony maintenance: help maintain the available mouse lines in the lab, including mouse husbandry, weaning the pups and update the mouse list.
- Genotyping: After weaning, label each mouse using a unique double labeling system (ear tag and numbering). Meanwhile, cut a small piece of tail for genotyping.
- PCR reaction: PCR was performed using extracted DNA from mouse tails. The PCR product would run for an agarose gel to determine the right genotyping.
- Responsible for changing special diet, either high fat diet or high fat diet plus doxycycline. During the diet change, weight the mouse and food, if necessary.
- After tissue collect, help extract RNA from the tissues and synthesize cDNA if needed.
- Real-time qPCR: if necessary, please help with RT-PCR to determine the abundance of a specific gene.
- Western blots: In case of urgent help, after harvesting the mouse tissues or cell line, please help extract protein and run for western blots.
- Help manage the lab, and routinely check the stock of each reagent and prevent the shortage of key supplies in the lab.
- Telephone different companies and request quote for different products and reagents. Then place the order in the ordering system.
Education:
Bachelor's in a basic science is required.
Experience:
None
The mission of UT Health San Antonio is to make lives better through excellence in education, research, health care and community engagement. Our vision is to be a world-class academic health center transforming health and health care for a dive...
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