Research Scientist I

Responsibilities

A Research Scientist I position focusing on translational discoveries in T cell exhaustion and cancer immunotherapy is available at the Kalia Lab. The project involves high dimensional immunomics approaches to make new discoveries in T cell exhaustion and memory, which feed seamlessly into our pipeline of next-generation engineered CAR T cell and checkpoint blockade immunotherapies. By delineating unifying themes in the generation and maintenance of memory and stem-like exhausted CD8 T cells, and developing therapeutic CAR-T cells that are long-lived, exhaustion-proof, and respond vigorously to checkpoint blockade immunotherapies, these studies aim to achieve metastasis-free and lifelong remission of intractable solid tumors such as pancreatic cancer, brain tumor, lung cancer, neuroblastoma and sarcoma.

Targeting key challenges in cancer immunotherapy, specific research areas of ongoing interest include:

• Programming of stem-like CAR T cells for enhanced function and responsiveness to checkpoint blockade therapy.

• Transcriptional and metabolic rewiring of therapeutic CAR T cells for on-demand switch in differentiation state using synthetic promoters.

• Attracting functionally potent therapeutic CAR-T cells into the TME.

• Tumor-targeted delivery of differentiation-tuned cytokines and costimulatory protein therapeutics by engineered T cells to remodel the TME.

• Delineating unifying themes in the generation and maintenance of memory and stem-like exhausted CD8 T cells for long-term sentinel function and prevention from relapse.

Requirements

Required Education/Experience: - Bachelor's Degree in biology, microbiology, immunology, molecular biology or biochemistry. - Prior experience in a laboratory setting. Required Credentials: - N/A Preferred: - Research experience in immunology, and tissue culture techniques is highly preferred.

• Experience with in vivo mouse experimentation.
• Experience with bioinformatics tools for large data set analysis.
• Experience in scientific writing
• Prior experience with tumor immunology in murine models
• Experience using Seahorse with metabolism related assays and metabolomics.
• Prior evidence of procuring any competitive funding (amount not important).
• Expertise in multi-color flow cytometry (>6-colors) and data analysis using FlowJo.
• Expertise in in vitro T cell assays
• Proficiency in molecular techniques such as DNA/RNA isolation, cloning, PCR,
• Quantitative RT-PCR, and retroviral transduction.

Min to Max Hourly Salary

$24.36 - $36.54 /hr

Min to Max Annual Salary

$50,668.80 - $76,003.20 /yr

Salary Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

The union pay ranges can be found on the Seattle Children's website here: WSNA - UFCW

Seattle Children's offers annual incentive pay based upon performance that is commensurate with the level of the position.

Disclaimer for Out of State Applicants

This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors.

Benefits Information

Seattle Children's offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Click here for more information.

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children's - to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.

Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine.

Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report's top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.

We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.

Covid-19 Vaccination

Seattle Children's requires that all employees be fully vaccinated against COVID-19. All offers are contingent and your vaccine status will be verified at onboarding.

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