Specialist – Microinjectionist

The UC Irvine Transgenic Mouse Facility (TMF) provides services for the design, production, breeding, storage and analysis of genetically modified mice. The facility supports investigators at UC Irvine as well as a large number of other universities and commercial entities throughout the USA that conduct biological and biomedical research.

Under the direction of the TMF Scientific Director, the successful applicant will perform duties required for production of genetically modified mice. The duties include:

• Handling, sexing and breeding of laboratory mice.
• Administration of hormones to mice to induce superovulation.
• Isolation, handling, microinjection, and micro-culture of pre-implantation embryos (single, 2 cell, 8 cell and blastocyst stages)
• Anesthesia and surgical transfer of pre-implantation embryos to the reproductive tract of female laboratory mice.
• Cryopreservation and reanimation of germplasm and preimplantation embryos, including IVF.
• Cesarian section delivery and fostering of neonatal mice.
• Biopsy of genetically modified mice.
• Extraction of genomic DNA from tissue biopsies.
• PCR based genotyping and management of multiple lines of genetically modified mice.
• Amplification and purification of DNA constructs.
• Preparation and QC of DNA constructs for microinjection.
• Provide training of microinjection, embryo transfer and vasectomy surgery techniques.

A terminal degree (or equivalent degree) or ten or more years of experience demonstrating expertise in the relevant specialization. At least three years of experience in microinjection with demonstrated ability to produce genetically modified mice in an efficient and reproducible manner.

• Curriculum Vitae - Your most recently updated C.V.

• Cover Letter

• Inclusive Excellence Activities Statement - Statement addressing how past and/or potential contributions to inclusive excellence will advance UCI's Commitment to Inclusive Excellence. See our guidance for writing an inclusive excellence activities statement.

• Sexual Misconduct, Harassment and Discrimination Declaration Form - All applicants of academic positions at the University of California, Irvine must download, complete, sign, and upload the following form with their application: Sexual Misconduct, Harassment and Discrimination Declaration Form

• Misc / Additional (Optional)

• 3 required (contact information only)

The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy.

As a condition of employment, you will be required to comply with University of California Policy on Vaccination Programs, as may be amended or revised from time to time. Federal, state, or local public health directives may impose additional requirements.

Additionally, you will be required to comply with the University of California Policy on Substance Abuse (APM-190)(Drug Free Environment) and University of California Smoke and Tobacco Free Environment policy. Depending on the job duties of your position, you may also be required to comply with the University of California’s Reporting Child Abuse and Neglect policy.