For the Senior Scientist, Antibodies role at Cradle, candidates need a PhD with at least 3 years of experience in the pharmaceutical industry. A deep understanding of antibody engineering, including expertise in expression, purification, screening, and characterization of various antibody formats, is essential. Experience with both in vitro and in vivo protein expression systems and core characterization methods is also required.

As a Senior Scientist, Antibodies at Cradle, you will lead the establishment of our mammalian cell lab, focusing on large-scale antibody libraries and plate-based assays. You'll drive antibody engineering initiatives, oversee CROs and collaborators, and ensure quality experimental data generation in partnership with our machine learning team. Mentoring and developing the lab team will also be key responsibilities.

At Cradle, we value continuous learning and growth, offering various training and career progression opportunities for the Senior Scientist, Antibodies. By fostering a collaborative environment and encouraging ongoing education, we support your journey in professional development within the innovative field of protein engineering.

Cradle prides itself on fostering a positive and flexible work culture for the Senior Scientist, Antibodies. We have a dedicated team of over 40 experienced professionals scattered across two locations, promoting open communication and collaboration while ensuring work-life balance. We encourage innovation and creativity, making it an exciting place to contribute meaningfully.

In the role of Senior Scientist, Antibodies at Cradle, you'll work on projects including the establishment of a mammalian cell laboratory and large-scale antibody libraries using techniques like FACS-based methods and yeast-two-hybrid. You will also engage in plate-based optimization and lead efforts to align experimental data with our machine learning initiatives.

At Cradle, we believe in contributing to the broader bioengineering community through our commitment to open science. As a Senior Scientist, Antibodies, you will play a key role in sharing our lab methods, tools, and developments, making a meaningful impact on the industry and fostering collaboration across research domains.

In the Senior Scientist, Antibodies position at Cradle, you can expect to utilize a range of tools and methods including plate-based assays, SPR, BLI, DLS, and ELISA for antibody characterization. You'll also be involved in advanced techniques focused on high-throughput screening and optimization.

When discussing your experience with antibody engineering, focus on your specific roles and contributions in past projects. Be sure to mention the types of antibodies you've worked with, expression systems used, and any successful outcomes or publications resulting from your work. Highlight your familiarity with both in vitro and in vivo methodologies.

In response to this question, outline your systematic approach to establishing lab workflows. Discuss your strategies for building efficient protocols, collaborating with team members, and integrating new technologies. Providing an example of a workflow you successfully developed in a previous role can strengthen your answer.

When asked about methodologies for antibody characterization, share your preferred techniques and explain the rationale behind your choices. Discuss how each method you prefer serves different objectives, such as specificity, affinity, or stability of antibodies, and provide insights into how these methodologies have contributed to successful projects.

To answer questions about mentoring, outline your approach to guiding junior team members by emphasizing hands-on training, encouraging open communication, and fostering a collaborative atmosphere. Provide examples of how you've mentored others in the past, perhaps through formal presentations, workshops, or individual guidance during experimental work.

In responding to this question, select a specific project that presented significant challenges and outline your thought process in navigating those obstacles. Discuss the techniques employed, the team collaboration needed, and the ultimate outcome. Pretend to convey resilience and adaptability through your approach.

When addressing your collaboration with machine learning teams, highlight how you communicate experimental needs and provide feedback on data generated. Describe any collaborative projects or integrations you participated in, emphasizing how this partnership led to improved antibody engineering outcomes.

For this question, elaborate on your experience employing high-throughput screening methods in antibody engineering. Discuss the specific techniques you have utilized, the scale of projects, and the impact of these methods on accelerating your research outcomes or enhancing the quality of data obtained.

In discussing this topic, talk about the various resources you utilize to keep abreast of the latest trends and breakthroughs in antibody engineering. This could include attending conferences, participating in workshops, engaging with the academic community through literature reviews, or being part of relevant online forums and groups.

When asked about strategic consideration in designing an antibody library, emphasize the importance of understanding the target and selecting appropriate scaffolds. Discuss how you evaluate potential variants and account for factors such as binding affinity and stability, ensuring that the final library aligns with the goals of your research.

In answering this question, emphasize the importance of clear communication across disciplines, particularly between experimentalists and computational teams. Discuss how you foster collaboration, share insights, and facilitate the interpretation of data to ensure the success of interdisciplinary projects in antibody engineering.