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Job Description

As an Associate Scientist, Next Generation Sequencing, you will deliver on the priorities of Global Biologics research and Biologics Engineering and Antibody Discovery organization by contributing to the discovery, optimization and engineering of the antibody and CAR-T cells by deep mining repertoire of therapeutic antibodies using NGS to accelerate programs to IND.

How you will contribute:               

• Generation of NGS library (RNAseq, amplicon), from single cell platform (10X, Rhapsody, Parse Bioscience, PIPseq, plate-based) or bulk B cells, perform library QC, library quantification and running/operating Miseq/Nextseq.

• Use molecular biology laboratory best practices for RNA isolation, cDNA synthesis, PCR amplification, SPRI bead based clean up and fragment analysis.

• Serve as a subject matter expert for library generation and work with a broad enterprise mindset.

• Strategize best workflow and design NGS primers to utilize NGS sequencing tools.

• Ability to manage several competing high priority project simultaneously.

• Document laboratory work using electronic laboratory notebook and follow the previously established protocol.

• Lead and evaluate the emerging single cell library generation and DNA sequencing platforms.

• Optimize, improve and troubleshoot NGS related workflows to get better sequencing data.

• Work independently with efficiency, agility, speed and collaborate to thrive in a high pace, high impact team environment.

Minimum Requirements/Qualifications: 

• MS with 3+ years and BS with 5+ years experience.

• A previous experience with building NGS libraries to deeply mine the antibody repertoires sequences is required.

• Excellent knowledge of details on the platform source of antibodies, repertoires (e.g. transgenic mice/display platforms; immune or naive repertoires) and their nuances is highly desirable.

• A strong background in molecular biology and able to work with RNA/DNA/Plasmids or cells as the starting material.

• Previous experience generating high quality NGS library as established by rigorous PCR condition optimization, QC process and troubleshooting.

• Experience performing DNA fragment purification using Blue Pippin and analyze the size using Tape Station 4200. Library quantification using qubit flex and qPCR.

• Knowledge of MiSeq/Nextseq sequencing operation and maintenance.

More about us:

At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs. Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work.

Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.

Takeda Compensation and Benefits Summary

We understand compensation is an important factor as you consider the next step in your career. We are committed to equitable pay for all employees, and we strive to be more transparent with our pay practices. 

For Location:

Boston, MA

U.S. Base Salary Range:

$96,600.00 - $151,800.00

EEO Statement

Takeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law.

Locations

Boston, MA

Worker Type

Employee

Worker Sub-Type

Regular

Time Type

Full time

Job Exempt

Yes

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