Cryo-FIB Scientist

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The national cryo-electron microscopy (cryo-EM) center, the Laboratory for BioMolecular Structure (LBMS) at Brookhaven National Laboratory, provides sample preparation, screening, and high-resolution data collection for both single-particle cryo-EM and cryo-electron tomography (cryo-ET) studies.

To expand LBMS’s cryo-ET capabilities, we are establishing a cryo-focused ion beam (cryo-FIB) platform to prepare thin lamellae of cells/tissues. In combination with cryo-ET, biological interactions in cellular environment and the organization/morphology of living cells can be visualized.

The LBMS is seeking an experienced scientist to join our team and support our efforts in expanding cryo-ET capabilities. The successful candidate will have a strong background in biology, biochemistry, and experience with cryo-ET techniques.

Essential Duties and Responsibilities:

• Use cryo-focused ion beam (cryo-FIB) to prepare thin lamellae for cryo-ET.

• Use cryo-ultramicrotome to prepare thin sections of tissues for cryo-ET.

• Use high-pressure freezer to prepare thick samples.

• Collect cryo-ET data and perform data analysis.

• Support the operation of LBMS.

Required Knowledge, Skills, and Abilities:

• Bachelor’s and 2+ years, or Master’s and 1+ year in the field of cell biology, plant biology, structure biology, immunology, biochemistry, biophysics, chemistry, cell biology, plant genetics, microbiology, virology, or a related field.

• Proven abilities for dissemination of research through publication in peer-reviewed journals.

• Ability to communicate effectively and constructively.

• Ability to work with flexible scheduling.

• Self-motivated and able to work both independently and as part of a team.

Preferred Knowledge, Skills, and Abilities:

• Bachelor’s and 5+ years, or Master’s and 3+ years, or Doctorate.

• Experience in use of a cryo-focused ion beam (cryo-FIB) for milling biological samples.

• Experience in use of a ultramicrotomes to cut biological tissues.

• Experience in use of high-pressure freezer to freeze thick samples for cryo-FIB or ultramicrotome.

• Experience in use of SEM and fluorescence microscopy.

• Experience in cryo-ET technique including sample preparation, data collection and analysis.

Brookhaven Laboratory is committed to providing fair, equitable and competitive compensation. This is a multi-level role and the salary range for this position is

$82,250.00 - $115,000.00 year. You will be placed at the level and salary commensurate with your experience.  Salary offers will be commensurate with the final candidate’s qualification, education and experience and considered with the internal peer group.

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Brookhaven National Laboratory (www.bnl.gov) delivers discovery science and transformative technology to power and secure the nation’s future. Brookhaven Lab is a multidisciplinary laboratory with seven Nobel Prize-winning discoveries, 37 R&D 100 Awards, and more than 70 years of pioneering research. The Lab is primarily supported by the U.S. Department of Energy’s (DOE) Office of Science. Brookhaven Science Associates (BSA) operates and manages the Laboratory for DOE. BSA is a partnership between Battelle and The Research Foundation for the State University of New York on behalf of Stony Brook University. BSA salutes our veterans and active military members with careers that leverage the skills and unique experience they gained while serving our country, learn more at BNL | Opportunities for Veterans at Brookhaven National Laboratory.

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