Senior Scientist I, CRISPR Platform Development

As a Senior Scientist I at Scribe Therapeutics, your primary responsibilities will include developing high throughput and multiplexed cell-based assays to analyze engineered CRISPR systems for both activity and specificity. You will also design and execute complex molecular biology workflows, assess protein variants, and collaborate with various interdisciplinary teams to create robust testing pipelines.

To qualify for the Senior Scientist I position at Scribe Therapeutics, candidates must hold a PhD in Biology, Bioengineering, Synthetic Biology, Genetics, or a related discipline, along with at least 8 years of relevant lab experience. Significant expertise in CRISPR genome editing mechanisms and practical experience with NGS workflows are essential.

In addition to the required skills, candidates for the Senior Scientist I role at Scribe Therapeutics are preferred to have experience in engineering CRISPR-Cas systems, protein engineering techniques, familiarity with CRISPR therapeutic approaches, and competency in automation of molecular biology workflows.

The work environment at Scribe Therapeutics is intellectually stimulating, collaborative, and fast-paced. As a Senior Scientist I, you will have the opportunity to work with a diverse group of experts in a culture that encourages rigorous scientific inquiry and the sharing of ideas.

At Scribe Therapeutics, professional growth is prioritized through continuous learning opportunities, the chance to take on leadership roles, and the ability to publish impactful research in peer-reviewed journals. The dynamic nature of the company allows for numerous avenues to enhance your career.

When answering, choose a specific project, describe the modifications made, the methodology used, and the outcomes. Highlight your role in the project and how it aligns with Scribe Therapeutics' research goals.

Discuss your systematic approach to assay design, including the identification of key parameters, selecting appropriate cell lines, and ensuring reproducibility. Highlight your experience with high throughput methods.

Share specific challenges encountered, such as specificity issues or delivery mechanisms. Discuss the strategies you employed to troubleshoot and the successful results achieved as a result of your interventions.

Talk about the types of NGS technologies you have used, how you implemented them in your previous roles, and the impact of NGS on your research outcomes. Include any relevant data analysis techniques.

Articulate your strategies for staying informed, such as subscribing to relevant journals, attending conferences, participating in workshops, or being part of scientific communities that focus on genetic engineering.

Provide details about specific projects where you worked alongside scientists from different fields. Focus on your communication style, conflict resolution, and how you ensured successful collaboration to meet project goals.

Express your enthusiasm for Scribe Therapeutics' mission, your alignment with its values, and how your skills and experiences make you a great fit for advancing their CRISPR platform development efforts.

Discuss the importance of guide RNA design, target site selection, and potential off-target effects. Highlight any specific examples from your work that demonstrate your understanding of these factors.

Provide an overview of the protein engineering techniques you’re familiar with, including deep mutational scanning and rational design. Mention specific projects where these techniques were leveraged successfully.

Discuss your project management style, the tools you utilize for prioritization, and how you align your tasks with team objectives while keeping timelines in mind. Emphasize communication and adaptability.